DE NOVO ASSEMBLY AND CHARACTERIZATION OF THE TRANSCRIPTOME, AND DEVELOPMENT OF SSR MARKERS IN WAX GOURD (BENICASA HISPIDA).

De novo assembly and characterization of the transcriptome, and development of SSR markers in wax gourd (Benicasa hispida).

BACKGROUND: Wax gourd is a widely used vegetable of Cucuribtaceae, and also has important medicinal and health values.However, the genomic resources of wax gourd were scarcity, and SEMET only a few nucleotide sequences could be obtained in public databases.METHODOLOGY/PRINCIPAL FINDINGS: In this study, we examined transcriptome in wax gourd.More th

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Construction of a Derivative of Agrobacterium tumefaciens C58 That Does Not Mutate to Tetracycline Resistance

Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance Neff D94BHM1N0B 90cm Black Stainless Steel Chimney Hood to this antibiotic as the selection marker.Such mutations are associated with a resistant gene unit, tetC58, tha

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